Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Peroxisome Deficiency in Cochlear Hair Cells Causes Hearing Loss by Deregulating BK Channels.

doi: 10.1002/advs.202300402

Figure Lengend Snippet: Figure 6. BK channel expression is decreased in cochlear hair cells of Atoh1-Pex5−/−mice. A) Schematic representation of the distribution of several important proteins involved in hair cell function. There are three important proteins in the inner hair cells: Glutamate, GluR, and BK channels, and there are three important proteins in the outer hair cells: PMCA2, Prestin, and KCNQ4. Among them, voltage-gated Ca2+ channels are distributed at the connection between the inner and outer hair cells at the afferent terminal. B) No significant difference was observed in the expression and distribution of PMCA2 between 1-month WT and Atoh1-Pex5−/−mice, n = 3 for each group. Scale bar 10 μm. C) No significant difference in the distribution and expression of Prestin protein in hair cells between 1-month WT and Atoh1-Pex5−/−mice, n = 5 for each group. Scale bar 10 μm. D) Immunofluorescence of KCNQ4 (red) from 1-month WT and Atoh1-Pex5−/−mice, n = 6 for each group. Scale bar 10 μm. E) Western blotting analysis of KCNQ4 and Prestin in the cochlea from 1-month WT and Atoh1-Pex5−/−mice. F) Quantification of KCNQ4 and Prestin levels from (E), n = 3 for each group. G) Immunofluorescence images of the BK 𝛼-subunit of the cochlea sensory epithelium from 1-month WT and Atoh1-Pex5−/- mice. The area selected by the white box is enlarged and displayed on the right side of the image. Scale bar 10 μm. H) BK-channel expression in hair cells of 1-month Atoh1-Pex5−/- mice was significantly reduced in apical, middle, and basal turns compared to 1-month WT mice, n = 3 for each group. Data represent the means ±SEM. “ns” represents not significant, *** p < 0.001, by two-tailed Student’s t-test.

Article Snippet: The following antibodies were used: PMP70 (ab3421, Abcam, 1:200), Parvalbumin (MAB1572, Sigma, 1:2000), Pex5 (NBP1-87185, Novus Biologicals, 1:200), Myo7a (25-6790, Proteus Biosciences, 1:200), Sox2 (ab97959, Abcam, 1:200), NF-200 (ab4680, Abcam, 1:200), 4-HNE (1:100, Abcam, ab46545), P-S6 (Ser 235/236) (4858T, CST, 1:200), PMCA2 (PA1-915, ThermoFisher Scientific, 1:200), Prestin (sc-2294, Santa Cruz, 1:200), KCNQ4 (SMC-309, StressMarq Biosciences, 1:200), KCNMA1 (APC-107, Alomone Labs, 1:200), TOMM20 (ab56783, Abcam, 1:200), otoferlin (ab53233, Abcam, 1:200), Ctbp2 (612 044, BD Transduction Laboratories, 1:200), GluR2 (MAB397, Millipore, 1:200), and Phalloidin-iFluor 594 (ab176757, Abcam, 1:2000).

Techniques: Expressing, Cell Function Assay, Western Blot, Two Tailed Test

Journal: Scientific Reports

Article Title: A Benzothiazole Derivative (5g) Induces DNA Damage And Potent G2/M Arrest In Cancer Cells

doi: 10.1038/s41598-017-02489-3

Figure Lengend Snippet: Evaluation of antiproliferative activity of 5g in various cancer cells. ( A ) 2-dimensional structure of 5g. ( B ) Antiproliferative activity of 5g (0, 1, 10, 50 and 100 µM at 48 h) was tested in Nalm6, Molt4, CEM, EAC, HCT116, T98G, MCF7 and HeLa cells using MTT assay. ( C ) Table showing observed GI50 values ± SEM of 5g in various cancer cell lines.

Article Snippet: Human cancer cell lines, MCF7 (human breast cancer), K562 (chronic myelogenous leukemia), Molt4 (acute lymphoblastic leukemia), CEM (T-cell leukemia), T98G (human glioblastoma multiforme tumor), HeLa (cervical cancer tumor) and EAC (mouse breast cancer) cells were purchased from National Centre for Cell Science, Pune, India and Nalm6 (B-cell leukemia) and REH (B-cell leukemia) cells were kind gifts from Dr. M.R.

Techniques: Activity Assay, MTT Assay